- The result means Bolt, 100 and 200m world record holder, finishes his career with 14 world medals to go with eight Olympic golds.
Usain Bolt's glittering career swansong came crashing to an end when he pulled up with a leg cramp on the anchor leg of the world 4x100m relay won by Britain on Saturday.
Bolt received the baton with Jamaica in third, but halfway down the finishing straight the towering sprinter pulled up clutching his left thigh, eventually doing a forward somersault to the ground, to gasps from the 60,000 sell out London Stadium.
The British quartet of Chijindu Ujah, Adam Gemili, Daniel Talbot and Nethaneel Mitchell-Blake claimed gold in 37.47 seconds, with the Justin Gatlin-led US four taking silver at 0.05sec and Japan a surprise bronze (38.04).
Jamaican team doctor Dr Kevin Jones said Bolt had suffered from "cramp in his left hamstring".
"But a lot of pain is from disappointment from losing the race," Jones said.
"The last three weeks have been hard for him, you know. We hope for the best for him."
Second leg runner Julian Forte added: "He didn't tell us exactly what happened but from what I saw, it looked like a strain or a cramp of some sort.
"He kept apologising to us but we told him there was no need to apologise — injuries are part of the sport."
Newly-crowned 110m hurdles champion Omar McLeod, Jamaica's lead-off runner, added: "Everybody was jelly, everybody was pumped. (Bolt's injury) just happened. Usain Bolt's name will always live on."
Bolt missed out on his bid to retain his 100m title earlier in the week, losing out to Gatlin and silver medallist Christian Coleman, who ran relay anchor for the Americans on Saturday.